https://peertube.lyclpg.itereva.pf/videos/watch/b33ddd26-72c7-4a71-982f-a27c77784afa The lateral mobility of membrane proteins can be measured in living cells by FRAP, which stands for fluorescence recovery after photobleaching. For this purpose, membrane proteins are often expressed as fusion proteins with the green fluorescent protein GFP and observed with a fluorescence microscope. A selected area of the cell is then bleached with a strong, computer controlled beam of laser light. Those membrane proteins that are not anchored and therefore can diffuse in the plane of the membrane, quickly exchange places with their neighbors and fill back in the bleached area. From the rate of this fluorescence recovery, the diffusion constant of the protein can be calculated. Here, GFP is fused to a membrane protein that lies in the membrane network of the endoplasmic reticulum. After bleaching, we observe quick recovery of the fluorescence, showing that the protein is very mobile in the plane of the membrane. The same experiment can be repeated using a protein that is firmly anchored and not free to diffuse. Here, we observe GFP fused to a protein of the inner nuclear membrane that binds tightly to the meshwork of the nuclear lamina. After photobleaching, no fluorescence recovery can be seen over the same time frame. Source : http://www.garlandscience.com/garlandscience_resources/resource_detail.jsf?landing=student&resource_id=9780815344544_CH11_QTM06 Thu, 04 Jun 2026 03:26:59 GMT https://validator.w3.org/feed/docs/rss2.html PeerTube - https://peertube.lyclpg.itereva.pf https://peertube.lyclpg.itereva.pf/client/assets/images/icons/icon-1500x1500.png https://peertube.lyclpg.itereva.pf/videos/watch/b33ddd26-72c7-4a71-982f-a27c77784afa All rights reserved, unless otherwise specified in the terms specified at https://peertube.lyclpg.itereva.pf/about and potential licenses granted by each content's rightholder.